Ramos-Hegazy, LaylaChakravarty, ShubhamAnderson, Gregory G.2022-05-052022-05-052020Ramos-Hegazy L, Chakravarty S, Anderson GG. Phosphoglycerate mutase affects Stenotrophomonas maltophilia attachment to biotic and abiotic surfaces. Microbes Infect. 2020;22(1):60-64. doi:10.1016/j.micinf.2019.08.001https://hdl.handle.net/1805/28854Stenotrophomonas maltophilia biofilm formation is of increasing medical concern, particularly for lung infections. However, the molecular mechanisms facilitating the biofilm lifestyle in S. maltophilia are poorly understood. We generated and screened a transposon mutant library for mutations that lead to altered biofilm formation compared to wild type. One of these mutations, in the gene for glycolytic enzyme phosphoglycerate mutase (gpmA), resulted in impaired attachment on abiotic and biotic surfaces. As adherence to a surface is the initial step in biofilm developmental processes, our results reveal a unique factor that could affect S. maltophilia biofilm initiation and, possibly, subsequent development.en-USPublisher PolicyBiofilmCystic fibrosisPhosphoglycerate mutaseStenotrophomonas maltophiliaArticle