DNA Methylation program in normal and alcohol-induced thinning cortex

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dc.contributor.authorÖztürk, Nail Can
dc.contributor.authorResendiz, Marisol
dc.contributor.authorÖztürk, Hakan
dc.contributor.authorZhou, Feng C.
dc.contributor.departmentAnatomy and Cell Biology, School of Medicineen_US
dc.date.accessioned2018-05-03T19:10:13Z
dc.date.available2018-05-03T19:10:13Z
dc.date.issued2017-05
dc.description.abstractWhile cerebral underdevelopment is a hallmark of fetal alcohol spectrum disorders (FASD), the mechanism(s) guiding the broad cortical neurodevelopmental deficits are not clear. DNA methylation is known to regulate early development and tissue specification through gene regulation. Here, we examined DNA methylation in the onset of alcohol-induced cortical thinning in a mouse model of FASD. C57BL/6 (B6) mice were administered a 4% alcohol (v/v) liquid diet from embryonic (E) days 7–16, and their embryos were harvested at E17, along with isocaloric liquid diet and lab chow controls. Cortical neuroanatomy, neural phenotypes, and epigenetic markers of methylation were assessed using immunohistochemistry, Western blot, and methyl-DNA assays. We report that cortical thickness, neuroepithelial proliferation, and neuronal migration and maturity were found to be deterred by alcohol at E17. Simultaneously, DNA methylation, including 5-methylcytosine (5mC) and 5-hydroxcylmethylcytosine (5hmC), which progresses as an intrinsic program guiding normal embryonic cortical development, was severely affected by in utero alcohol exposure. The intricate relationship between cortical thinning and this DNA methylation program disruption is detailed and illustrated. DNA methylation, dynamic across the multiple cortical layers during the late embryonic stage, is highly disrupted by fetal alcohol exposure; this disruption occurs in tandem with characteristic developmental abnormalities, ranging from structural to molecular. Finally, our findings point to a significant question for future exploration: whether epigenetics guides neurodevelopment or whether developmental conditions dictate epigenetic dynamics in the context of alcohol-induced cortical teratogenesis.en_US
dc.eprint.versionAuthor's manuscripten_US
dc.identifier.citationÖztürk, N. C., Resendiz, M., Öztürk, H., & Zhou, F. C. (2017). DNA Methylation program in normal and alcohol-induced thinning cortex. Alcohol (Fayetteville, N.Y.), 60, 135–147. https://doi.org/10.1016/j.alcohol.2017.01.006en_US
dc.identifier.issn0741-8329en_US
dc.identifier.urihttps://hdl.handle.net/1805/16034
dc.language.isoen_USen_US
dc.publisherElsevieren_US
dc.relation.isversionof10.1016/j.alcohol.2017.01.006en_US
dc.relation.journalAlcohol (Fayetteville, N.Y.)en_US
dc.rightsPublisher Policyen_US
dc.sourcePMCen_US
dc.subjectEpigeneticsen_US
dc.subjectFetal Alcohol Spectrum Disorders (FASD)en_US
dc.subjectNeurodevelopmental deficiten_US
dc.subjectNeuroepigeneticsen_US
dc.subjectNeurogenesisen_US
dc.titleDNA Methylation program in normal and alcohol-induced thinning cortexen_US
dc.typeArticleen_US
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