Creating class I MHC-null pigs using guide RNA and the Cas9 endonuclease

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dc.contributor.authorReyes, Luz M.
dc.contributor.authorEstrada, Jose L
dc.contributor.authorWang, Zheng Yu
dc.contributor.authorBlosser, Rachel J.
dc.contributor.authorSmith, Rashod F.
dc.contributor.authorSidner, Richard A.
dc.contributor.authorParis, Leela L.
dc.contributor.authorBlankenship, Ross L.
dc.contributor.authorRay, Caitlin N.
dc.contributor.authorMiner, Aaron C.
dc.contributor.authorTector, Matthew
dc.contributor.authorTector, A. Joseph
dc.contributor.departmentSurgery, School of Medicineen_US
dc.date.accessioned2018-08-29T18:08:18Z
dc.date.available2018-08-29T18:08:18Z
dc.date.issued2014-12-01
dc.description.abstractPigs are emerging as important large animal models for biomedical research, and they may represent a source of organs for xenotransplantation. The MHC is pivotal to the function of the immune system in health and disease, and it is particularly important in infection and transplant rejection. Pigs deficient in class I MHC could serve as important reagents to study viral immunity as well as allograft and xenograft rejection. In this study, we report the creation and characterization of class I MHC knockout pigs using the Cas9 nuclease and guide RNAs. Pig fetal fibroblasts were genetically engineered using Cas9 and guide RNAs, and class I MHC(-) cells were then used as nuclear donors for somatic cell nuclear transfer. We produced three piglets devoid of all cell surface class I proteins. Although these animals have reduced levels of CD4(-)CD8(+) T cells in peripheral blood, the pigs appear healthy and are developing normally. These pigs are a promising reagent for immunological research.en_US
dc.eprint.versionAuthor's manuscripten_US
dc.identifier.citationReyes, L. M., Estrada, J. L., Wang, Z. Y., Blosser, R. J., Smith, R. F., Sidner, R. A., … Tector, A. J. (2014). Creating Class I MHC Null Pigs Using gRNA and the Cas9 Endonuclease. Journal of Immunology (Baltimore, Md. : 1950), 193(11), 5751–5757. http://doi.org/10.4049/jimmunol.1402059en_US
dc.identifier.urihttps://hdl.handle.net/1805/17229
dc.language.isoen_USen_US
dc.publisherAmerican Association of Immunologistsen_US
dc.relation.isversionof10.4049/jimmunol.1402059en_US
dc.relation.journalJournal of Immunologyen_US
dc.rightsPublisher Policyen_US
dc.sourcePMCen_US
dc.subjectSwine leucocyte antigen class Ien_US
dc.subjectMHCen_US
dc.subjectSwineen_US
dc.subjectCRISPR/Cas9en_US
dc.subjectGene knockouten_US
dc.subjectSLA deficiencyen_US
dc.titleCreating class I MHC-null pigs using guide RNA and the Cas9 endonucleaseen_US
dc.typeArticleen_US
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