Methane, arsenic, selenium and the origins of the DMSO reductase family

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dc.contributor.authorWells, Michael
dc.contributor.authorKanmanii, Narthana Jeganathar
dc.contributor.authorAl Zadjali, Al Muatasim
dc.contributor.authorJanecka, Jan E.
dc.contributor.authorBasu, Partha
dc.contributor.authorOremland, Ronald S.
dc.contributor.authorStolz, John F.
dc.contributor.departmentChemistry and Chemical Biology, School of Scienceen_US
dc.date.accessioned2020-12-07T16:50:18Z
dc.date.available2020-12-07T16:50:18Z
dc.date.issued2020-07-02
dc.description.abstractMononuclear molybdoenzymes of the dimethyl sulfoxide reductase (DMSOR) family catalyze a number of reactions essential to the carbon, nitrogen, sulfur, arsenic, and selenium biogeochemical cycles. These enzymes are also ancient, with many lineages likely predating the divergence of the last universal common ancestor into the Bacteria and Archaea domains. We have constructed rooted phylogenies for over 1,550 representatives of the DMSOR family using maximum likelihood methods to investigate the evolution of the arsenic biogeochemical cycle. The phylogenetic analysis provides compelling evidence that formylmethanofuran dehydrogenase B subunits, which catalyze the reduction of CO2 to formate during hydrogenotrophic methanogenesis, constitutes the most ancient lineage. Our analysis also provides robust support for selenocysteine as the ancestral ligand for the Mo/W atom. Finally, we demonstrate that anaerobic arsenite oxidase and respiratory arsenate reductase catalytic subunits represent a more ancient lineage of DMSORs compared to aerobic arsenite oxidase catalytic subunits, which evolved from the assimilatory nitrate reductase lineage. This provides substantial support for an active arsenic biogeochemical cycle on the anoxic Archean Earth. Our work emphasizes that the use of chalcophilic elements as substrates as well as the Mo/W ligand in DMSORs has indelibly shaped the diversification of these enzymes through deep time.en_US
dc.identifier.citationWells, M., Kanmanii, N. J., Al Zadjali, A. M., Janecka, J. E., Basu, P., Oremland, R. S., & Stolz, J. F. (2020). Methane, arsenic, selenium and the origins of the DMSO reductase family. Scientific Reports, 10(1), 10946. https://doi.org/10.1038/s41598-020-67892-9en_US
dc.identifier.issn2045-2322en_US
dc.identifier.urihttps://hdl.handle.net/1805/24539
dc.language.isoen_USen_US
dc.publisherNature Publishing groupen_US
dc.relation.isversionof10.1038/s41598-020-67892-9en_US
dc.relation.journalScientific Reportsen_US
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.sourcePMCen_US
dc.subjectBiochemistryen_US
dc.subjectComputational biology and bioinformaticsen_US
dc.subjectEvolutionen_US
dc.subjectMicrobiologyen_US
dc.subjectBiogeochemistryen_US
dc.titleMethane, arsenic, selenium and the origins of the DMSO reductase familyen_US
dc.typeArticleen_US
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