Epigenomic profiling of retinal progenitors reveals LHX2 is required for developmental regulation of open chromatin

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dc.contributor.authorZibetti, Cristina
dc.contributor.authorLiu, Sheng
dc.contributor.authorWan, Jun
dc.contributor.authorQian, Jiang
dc.contributor.authorBlackshaw, Seth
dc.contributor.departmentMedical and Molecular Genetics, School of Medicineen_US
dc.date.accessioned2019-08-14T15:51:56Z
dc.date.available2019-08-14T15:51:56Z
dc.date.issued2019-04-25
dc.description.abstractRetinal neurogenesis occurs through partially overlapping temporal windows, driven by concerted actions of transcription factors which, in turn, may contribute to the establishment of divergent genetic programs in the developing retina by coordinating variations in chromatin landscapes. Here we comprehensively profile murine retinal progenitors by integrating next generation sequencing methods and interrogate changes in chromatin accessibility at embryonic and post-natal stages. An unbiased search for motifs in open chromatin regions identifies putative factors involved in the developmental progression of the epigenome in retinal progenitor cells. Among these factors, the transcription factor LHX2 exhibits a developmentally regulated cis-regulatory repertoire and stage-dependent motif instances. Using loss-of-function assays, we determine LHX2 coordinates variations in chromatin accessibility, by competition for nucleosome occupancy and secondary regulation of candidate pioneer factors.en_US
dc.identifier.citationZibetti, C., Liu, S., Wan, J., Qian, J., & Blackshaw, S. (2019). Epigenomic profiling of retinal progenitors reveals LHX2 is required for developmental regulation of open chromatin. Communications biology, 2, 142. doi:10.1038/s42003-019-0375-9en_US
dc.identifier.urihttps://hdl.handle.net/1805/20362
dc.language.isoen_USen_US
dc.publisherSpringer Natureen_US
dc.relation.isversionof10.1038/s42003-019-0375-9en_US
dc.relation.journalCommunications Biologyen_US
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/us/*
dc.sourcePMCen_US
dc.subjectEpigenomicsen_US
dc.subjectDevelopmental neurogenesisen_US
dc.titleEpigenomic profiling of retinal progenitors reveals LHX2 is required for developmental regulation of open chromatinen_US
dc.typeArticleen_US
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