Hepatic lipid peroxidation and cytochrome P-450 2E1 in pediatric nonalcoholic fatty liver disease and its subtypes

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dc.contributor.authorBell, Lauren N.
dc.contributor.authorMolleston, Jean P.
dc.contributor.authorMorton, Michael J.
dc.contributor.authorKlipsch, Ann
dc.contributor.authorSaxena, Romil
dc.contributor.authorVuppalanchi, Raj
dc.contributor.authorChalasani, Naga
dc.contributor.departmentDepartment of Medicine, IU School of Medicineen_US
dc.date.accessioned2017-06-29T20:05:33Z
dc.date.available2017-06-29T20:05:33Z
dc.date.issued2011-10
dc.description.abstractGOAL: To compare hepatic lipid peroxidation and cytochrome P-450 2E1 (CYP2E1) protein content in liver biopsies from children with nonalcoholic fatty liver disease (NAFLD) and 2 control groups. BACKGROUND: Elevated hepatic lipid peroxidation resulting from increased hepatic CYP2E1 enzyme activity is involved in the pathogenesis of NAFLD and nonalcoholic steatohepatitis (NASH) in adults, but studies in children are lacking. STUDY: Liver biopsies from 59 children with NAFLD (49 with NASH), 10 children with normal liver histology, and 9 children with mild chronic hepatitis C (HCV) infection were examined. Hepatic malondialdehyde (a measure of lipid peroxidation) levels and CYP2E1 protein content were quantitated, as a percentage of the total area, by immunohistochemical staining of liver biopsy material followed by digital image quantitation. RESULTS: Lipid peroxidation was significantly greater in NAFLD liver biopsies (46.7 ± 20.8%) compared with biopsies from children with normal liver histology (7.6 ± 9.4%; P<0.001) or HCV infection (7.7 ± 7.6%; P<0.001). However, hepatic CYP2E1 expression was not different across the NAFLD, normal liver histology, and HCV groups (60.7 ± 8.7%, 53.5 ± 10.7%, and 60.0 ± 11.9%, respectively; P=0.116). Among children with NAFLD, lipid peroxidation and CYP2E1 protein content did not differ between biopsies with and without NASH. Body mass index was independently associated with hepatic lipid peroxidation levels (r=0.549; P<0.001). CONCLUSIONS: Hepatic lipid peroxidation is increased in children with NAFLD but this is not related to hepatic CYP2E1 expression. No difference in lipid peroxidation in pediatric NAFLD versus NASH argues against a role in disease progression.en_US
dc.eprint.versionAuthor's manuscripten_US
dc.identifier.citationBell, L. N., Molleston, J. P., Morton, M. J., Klipsch, A., Saxena, R., Vuppalanchi, R., & Chalasani, N. (2011). Hepatic Lipid Peroxidation and Cytochrome P-450 2E1 in Pediatric Nonalcoholic Fatty Liver Disease and its Subtypes. Journal of Clinical Gastroenterology, 45(9), 800–807. http://doi.org/10.1097/MCG.0b013e31821377e4en_US
dc.identifier.urihttps://hdl.handle.net/1805/13272
dc.language.isoen_USen_US
dc.publisherWolters Kluweren_US
dc.relation.isversionof10.1097/MCG.0b013e31821377e4en_US
dc.relation.journalJournal of Clinical Gastroenterologyen_US
dc.rightsPublisher Policyen_US
dc.sourcePMCen_US
dc.subjectNonalcoholic steatohepatitisen_US
dc.subjectSteatosisen_US
dc.subjectFibrosisen_US
dc.subjectOxidative stressen_US
dc.subjectChildrenen_US
dc.titleHepatic lipid peroxidation and cytochrome P-450 2E1 in pediatric nonalcoholic fatty liver disease and its subtypesen_US
dc.typeArticleen_US
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